RP-HPLC法同时测定金莲花中3种碳苷黄酮的含量

Content determination of three flavonoid C-glycosides in Trollius chinensis by RP-HPLC

目的建立金莲花药材中3种碳苷黄酮含量同时测定的方法,以比较不同产地金莲花药材及不同部位中3种成分的含量。方法采用RP-HPLC法。色谱柱:Shim-pack C18柱(250 mm×4.6 mm,5μm),流动相:乙腈体积分数为0.35%的磷酸溶液(体积比为16∶84),流速:1.0 mL·min-1,检测波长:345 nm,柱温:25℃。结果荭草苷、牡荆苷、荭草素-2″-O-β-L-半乳糖苷能够达到基线分离;荭草苷在9.94～99.40 mg·L-1、牡荆苷在2.49～24.90 mg·L-1、荭草素2″OβL半乳糖苷在10.64～106.40 mg·L-1内线性关系良好。结论本方法为金莲花药材的综合利用和资源开发提供了参考,有利于控制金莲花药材的质量。

Objective To establish a high performance liquid chromatographic method for analysis of three flavonoid C-glycosides from different habitats and different parts of TroUius chinensis. Methods Shim-pack C18 column（250 mm x 4.6 mm,5 μm）was used with a mixture of acetonitrile-0. 35% phosphoric acid（ V： V = 16：84 ） as the mobile phase. The flow rate was 1.0 mL. rain - 1, detection wavelength was set at 345 nm, column temperature was set at 25 ℃. Results The linear range of orientin, vetixin and 2＂-O-β-L-galactopyranosyl orientin were 9.94-99.40,2. 49-24.90 and 10. 64- 106. 40 mg.L-1 ,respectively. Conclusions The method is simple, accurate and sensitive with good reproducibility. It can provide references for quality control and resources development of the medicinal materials.