羟丙基-β-环糊精为手性选择剂分离6种手性药物

Enantiomeric separation of six chiral drugs with hydroxypropyl-β-cyclodextrin as a chiral selector

目的以羟丙基-β-环糊精(HP-β-CD)为手性选择剂,利用毛细管电泳法分离6种药物对映体。方法采用未涂层熔融石英毛细管柱,磷酸二氢钠缓冲溶液,分离电压20 kV,考察了背景电解质pH值、环糊精浓度、缓冲盐浓度对对映体分离的影响。结果在最优的电泳条件下,托吡卡胺、氨氯地平、尼卡地平、特布他林、盐酸曲马多和盐酸酚苄明的分离度分别为2.6、3.7、1.3、4.1、2.5和1.8。结论所建立的方法适于托吡卡胺、氨氯地平、特布他林、盐酸曲马多和盐酸酚苄明的对映体分离。

Objective To separate the enantiomers of six drugs by capillary electrophoresis with hydroxyprop- yl-β-cyclodextrin（HP-fl-CD） as a chiral selector. Methods The separation of the enantiomers was performed in an untreated fused-silica capillary tube, in the buffer of NaH2PO4 with the voltage of 20 kV. The influences of buffer pH, HP-β-CD concentration, buffer concentration on separation were investigated. Results Under the optimal conditions, the resolutions of tropicamide, amlodipine, nicardipine, terbutaline, tramadol hydrochloride and phenoxybenzamine hydrochloride were Conclusions The method is effective for enantioseparation hydrochloride and Dhenoxvbenzamine hvdrochloride 2.6,3.7,1.3,4. 1,2. 5 and 1.8, respectively. of tropicamide, amlodipine, terbutaline, tramadol