荆豆凝集素修饰的牛血清白蛋白脂质体的免疫学性质

Imuune evaluation of ulex europaeus agglutinin I modified liposomes loading bovine serum albumin

目的对荆豆凝集素修饰的牛血清白蛋白脂质体进行免疫学评价。方法 3组小鼠分别于0、7、14、21 d口服免疫牛血清白蛋白溶液(bovine serum albumin,BSA)、BSA脂质体及荆豆凝集素修饰的BSA脂质体,建立ELISA法测定小鼠在7、14、21、28、35及42 d时体内产生的系统及黏膜免疫应答。结果与BSA溶液组相比,将抗原包裹于脂质体、尤其是荆豆凝集素修饰的脂质体中免疫小鼠后,可在小鼠血清及小肠分泌液中检测到较高的IgG及IgA水平。其中,免疫后42 d,凝集素修饰脂质体组的IgG和IgA水平分别为BSA溶液组产生相应抗体值的4.33倍和4.74倍。结论荆豆凝集素修饰脂质体口服免疫小鼠后可同时诱导机体产生黏膜及系统免疫应答,可以作为口服疫苗的载体。

Objective To establish the immune evaluation method of ulex europaeus agglutinin I modified liposomes loading bovine serum albumin. Methods Three groups of mice were respectively immunized by BSA solution ,LIP and UEAI-LIP. All animals received four immunization doses at 0,7,14 and 21 day and samples were collected after 7,14,21,28,35 and 42 days of primary immunization. Results Results indicate that antigen encapsulated in liposomes, especially the UEAI-modified ones, was favorable for inducing immune response. At 42 days after the first immunization, the highest IgG and IgA antibody levels produced by UEAI-LIP occurred, respectively showing 4.33-fold and 4. 74-fold higher levels compared to those of the groups receiving BSA alone. Conclusions UEAI-modified liposomes has high potential when used as carrier for oral vaccines.