GC-MS法分析豫南黑猪肌肉中脂肪酸组成及含量

Determination of Yunan Black Pigs Muscle Fatty Acid Composition and Content by GC-MS

为探讨豫南黑猪的种质特性,对豫南黑猪背最长肌脂肪酸组成及含量进行分析测定。采用甲醇-氯仿提取背最长肌脂肪酸,用KOH-甲醇进行脂肪酸甲酯化,利用GC-MS法对豫南黑猪背最长肌脂肪酸组成及含量进行测定。定性定量分析结果表明,豫南黑猪背最长肌脂肪酸至少有18种,含量占优势的脂肪酸为C18︰1、C16︰0、C18︰0、C18︰2、C16︰1、C14︰0;饱和与不饱和脂肪酸之比为0.82︰1。本试验分析确定了豫南黑猪背最长肌脂肪酸的组成及含量,为进一步研究肉脂肪酸与人体健康之间关系打下了基础。

Determine longissimus dorsi muscle fatty acid composition and content of Yunan Black Pigs. Lipids were extracted from the longissimus Dorsi Muscle with methanol/chloroform and were methylesterified using methanol/potassium hydroxide. Fatty acid methyl esters were then assayed by GC-MS Method. Qualitative analysis results showed that there were 18 kinds of fatty acids in longissimus dorsi muscle, with major fatty acids as C18：1, C16： 0, C18：0,C18：2, C16：1, C14：0. The ratio of saturated fatty acids to unsaturated fatty acids in rat cerebral cortex was 0.82 ： 1. This study provided data on fatty acid composition and content of Yunan black pigs＇ longissimus dorsi muscle, which laid the foundation for exploring the relationship between fatty acids and human health.