用狂犬病毒单克隆抗体2E9在小鼠引发狂犬病“早期死亡”综合征的实验研究

STUDY ON THE "EARLY DEATH" SYNDROME IN MICE BY USING MONOCLONAL ANTIBODIES TO RABIES VIRUS

单克隆抗体9E6和2E9都是针对狂犬病毒糖蛋白(61kD)的两个具有中和活性的单克隆抗体,但它们分别针对两个不同的抗原位点。当它们被动转移给小白鼠后,却产生完全相反的作用。在狂犬病小鼠动物模型上,9E6抗体的被动转移可使小鼠产生对狂犬病毒攻击的保护性免疫。而在相同实验条件下,2E9的被动转移却使小鼠在受狂犬病毒攻击后产生“早期死亡”综合征。而且这种“早期死亡”综台征的发生还依赖于被动转移抗体2E9的浓度,经1:30和1:300稀释的抗体可引起早期死亡综合征,而1:3和1:3000稀释者则不能。结果提示,早期死亡综合征的发生与病毒抗原上的某个决定簇相关,并由针对此决定簇的抗体所介导。

Monoclonal antibodies, 9E6 and 2E9 are directed against two different epitopes on the surface of 61kD glycoprotein of rabies virus ( RV ) . Passively transferred 2E9 to mice before or after rabies virus challenge could cause early death of some challenged mice. Also, its occurence depends on the concentration of McAb and the administration time.At 5 days before challenge with RV, 2E9 ( 1 : 300 ) induced early death syndrome. On 5 and 2 days before challenge or 2 days after challenge, 2E9 ( 1 : 30 ) consistently induced a early death syndrome. However, under the same condition 9E6 did not induce this phenomenon, but produced a protective effect against rabies virus. The results suggested that the occurrence of early death is related to a given epitope on the antigen and is induced by the antibody which reactes with this epitope. The concentration of the passively transferred antibody and the administration time are also important factors.