人转化生长因子β型基因在逆转录病毒载体的克隆及其细胞生长抑制作用

CLONING OF HUMAN TRANSFORMING GROWTH FACTOR TYPE-β IN RETROVIRUS VECTOR AND ITS CELLULAR GROWTH INHIBITION EFFECT

将人转化生长因子β型(HTGF-β)基因克隆到逆转录病毒载体pDO R-Neo中,构建一株重组质粒(pDOT)。然后将pDOT转染到逆转录病毒的包装细胞(ψ2和pA317细胞),组装成为一个携带HTGF-β基因的重组病毒。对重组病毒的滴度,所携带的HTGF-β基因的表达,对感染的NIH 3T3细胞生长的抑制作用进行了初步研究。

Human transforming growth factor type-β ( HTGF-β ) was cloned into retrovirus vector pDO R-Neo,obtaining a recombinant plasmid pDOT, then the pDOT was transfected into the packaging cell lines, ψ2 and PA317, producing the recombinant retrovirus which carried the HTGF-β gene. The infectious titer of the recombinant virus, the expression of HTGF-β as shown by the growth inhibition effect in infected NIH3T3 cell line were studied.