HPLC等度洗脱法同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量

Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution

目的:建立同时测定银黄薄膜衣片中绿原酸和黄芩苷含量的方法。方法:采用HPLC等度洗脱法,C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水-磷酸(45∶55∶0.2),流速1.0 mL.min-1,柱温30℃,检测波长328 nm,进样量20μL。结果:绿原酸和黄芩苷在2.200～44.00 mg.L-1和24.20～242.0 mg.L-1峰面积与进样量呈良好的线性关系,平均回收率分别为99.63%(RSD 1.8%,n=9)和99.72%(RSD 1.9%,n=9)。结论:该方法简便、准确,可同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量。

Objective： To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coa- ted tablets. Method： HPLC isocratic elution was adopted, with the C18 column （4. 6 mm × 250 mm, 5 μm） as the chromatographic column, methanol-water-phosphoric acid （45： 55： 0. 2） as the mobile phase. The flow rate was set at 1.0 mL · min-1, and column temperature was set at 30 ℃. The detection wavelength was 328 rim, and the sample size was 20 μL. Result： At concentrations ran- ging between 2. 200-44. 00,24. 20-242. 0 mg · L^-1, peak area of chlorogenic acid and baicalin showed good linear relationship, the av- erage recoveries were 99. 63 % （ RSD 1.8%, n = 9） and 99. 72% （ RSD 1.9%, n = 9 ）, respectively. Conclusion： The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.