高效液相色谱蒸发光散射检测法测定闹羊花中闹羊花毒素Ⅱ的含量

Quantification of rhodojaponinⅡin Rhododendri mollis flos by HPLC-ELSD

目的:建立闹羊花中闹羊花毒素Ⅱ的HPLC测定方法。方法:以Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm)色谱柱为固定相,以水-乙腈为流动相,梯度洗脱,流速0.8 mL.min-1,蒸发光散射检测器检测。结果:闹羊花毒素Ⅱ在0.190 4～1.904 0μg范围内,进样量的对数值与峰面积的对数值之间有良好的线性关系(R2=0.999),平均回收率为98.6%;测得10批闹羊花药材中闹羊花毒素Ⅱ的含量为0.057 7%～0.136 7%。结论:所建立的含量测定方法简便、快速、重复性好,可用于闹羊花中毒性成分闹羊花毒素Ⅱ的含量测定。

Objective ： To establish an HPLC method for the determination of a toxic compound, rhodojaponin Ⅱ, in Rhododendri mollis flos. Methods： Rhodojaponin II was separated and quantified upon an Agilent ZOR- BAX SB-C18 column （250 mm × 4.6 mm, 5 μm） using gradient elution of water-acetonitrile at the flow rate of 0. 8 mL· min^-1 with an evaporative light scattering detector. Results ： Within the range of 0. 190 4 - 1. 904 0μg, the double logarithmic plots of the peak area versus the injection amount of rhodojaponin lI showed good correlation （R2 =0.999） , and the mean recovery was 98.6%. The content of rhodojaponin I1 varied from 0. 057 7% to 0. 136 7% in 10 batches of Rhododendri mollisflos. Conclusion： The established HPLC method proved to be sim- ple and rapid, and can be applied to the determination of rhodojaponin 1I in Rhododendri mollls flos.