细菌硝基还原酶的结构与催化机制

Research progress of the structure and mechanism of bacterial nitroreductases

细菌硝基还原酶(nitroreductase,NTR)属于依赖黄素单核苷酸(flavin mononucleotide,FMN)的硝基还原酶超家族,通常以二聚体形式存在。它们可利用还原型烟酰胺腺嘌呤二核苷酸(磷酸)(reduced nicotinamide adenine dinucleotide(phosphate),NAD(P)H)作为电子供体,催化多种外源硝基芳香族、醌类和黄素类化合物的还原反应,在药物的激活和解毒机制中发挥重要的作用。以研究得较为透彻的大肠杆菌NTR为代表,总结了近几年细菌NTR在结构特征、构象变化及催化特性等方面的最新研究进展。最后,对细菌NTR的研究方向进行了展望。

Bacterial nitroreductases （NTRs） belong to FMN-dependent nitroreductase-like superfamily, and are mostly dimeric. They are NAD（P）H-dependent cytosolic enzymes with broad substrate spectrum including quinines, flavins and nitroaromatic compounds, and play important roles in drug activation and detoxication. Taking the well-characterized E.coli NTR NfsB and other bacteria NTR as examples, we summarized the recent research progress on the structural, conformational changes and catalytic properties of NTR. We also discussed the research perspectives in brief.