肺癌细胞粘弹特性与超微结构的相关性研究

Investigation on the correlation of the viscoelastic properties and ultrastructure of lung cancer cells

目的：肺癌是高恶性和高死亡率的肿瘤之一，作者力图从生物力学角度揭示肺癌细胞侵润转移的某些生物学特性。材料与方法：本文结合超微结构观察,采用微管吸吮技术定量测定体外培养的低转移人肺腺癌(PAa)细胞和高转移人肺巨细胞癌(PG)细胞在长春新碱(VCR)作用下的粘弹特性。结果：肺癌细胞粘弹性,在未使用VCR前为PAa细胞大于PG细胞；随VCR的使用和剂量增加,其在PAa细胞呈浓度依赖性下降,在PG细胞则表现为低VCR浓度(0．10 μg／ml)时降低而后以弹性系数K1,K2为主的增高。超微结构观察见PAa细胞微管、微丝稀疏,VCR作用后进一步减少；PG细胞微管、微丝减缺,VCR作用后分裂期形态表现多见。结论：细胞内微管、微丝等细胞骨架成份的改变与癌细胞运动迁徙能力有关,VCR等细胞骨架干扰剂的抗肿瘤作用可能主要通过调控癌细胞的增殖分化信息来影响癌细胞生物学特性及侵润转移能力。

： Purpose：Lung cancer was one of the neoplasms with high malignancy and mortality fate．The cytoskeleton structure were strong associated with their invasion and metastasis,the authors will try to reveal the relevant biomechanical and biorheological manifestations of human lung cancer．Methods：Using micropipette aspiration technique,authors investigated quantitatively the effect of vincristine(VCR,0．10～2．00 μg／ml) on the viscoelastic properties of high metastatic human giant cell carcinoma(PG) cells as well as low metastatic adenocarcinoma(PAa) cells of lung based on cell culture in vitro,Viscoelasticity was analyzed by using the special three－element standard linear solid model．Result：No VCR,the elastic coefficients K1 and K2 as well as viscous coefficient μ of PAa were bigger than that of PG cells． Treatment with VCR, the values of K1,K2 and μ of PAa cells decreased only as concentration increased,but on PG cells, the most viscoelastic coefficient values decrease in the presence of 0．10 μg／ml VCR, then turn to increase(esp．K1,K2)as concentration increased．By using electromicroscope,authors found that the microtubules and microfilments were sparse random in the PAa cells, and they were diminished in PG cells． After treated with VCR, they cut down seriously in PAa cells,and manifestations of mitotic arrest of cell cycle were increased unusually in PG cells．Conclusion：The cytoskele－ton structure components changes were ability related to the migration and movement of the tumor cells．The anti－neoplasm actions of cytoskeleton interferents such as VCR might be realized by regulating mainly the message of tumor cells growth and differentiation．