小麦地方品种淀粉颗粒结合蛋白及淀粉含量差异研究

Starch Granule-Associated Proteins and Starch Content in Wheat Landrace

淀粉颗粒结合蛋白包含了多种淀粉生物合成的关键酶,对作物淀粉品质有重要影响。本研究利用1D-SDS-PAGE,分离了74份四川、西藏及云南毗邻地区小麦的淀粉颗粒结合蛋白,对突变材料进行了分子标记检测,对总淀粉和直链淀粉含量差异进行了比较。发现供试材料中,在分子量57～130 kDa区域共有9种不同的蛋白条带。其中,2个条带可能为新的淀粉颗粒结合蛋白;存在12份Wx-B1缺失的自然突变体和3份稀有的SGP-B1缺失突变体;筛选到直链淀粉含量超过30%的材料2份,直链淀粉含量为15%左右的材料10份。这些材料为小麦淀粉品质改良及淀粉生化合成机理研究提供了基础。

The majority of Starch granule-associated proteins（SGAPs）is believed to correspond to starch biologi- cal enzymes and thus is important to starch quality in the breeding programms. In this study,SGAPs of 74 indigenous wheat lines from the adjacent areas of Sichuan,Tibet, and Yunnan of China using 1D-SDS-PAGE were separated. A to- tal of nine bands with molecular weights 57 - 130 kDa were observed,of which two bands was not reported in previous study and might represent novel proteins. Twelve and three lines were respectively identified to have a deletion of Wx- B1 and SGP-B1 （SSII）. The amylose content of the 74 lines varied from 14. 73% to 40. 73% ,with an average of 20. 39%. Among them,10 lines showed a low level of 15% while 2 lines, showed a high level of over 30%. These lines are very important for further study on the starch synthesis mechanism and improvement of starch quality.