适合于胞质基因组扩增的红麻成熟叶片DNA提取改良方法

The Improvement Method of Extraction DNA in Mature Kenaf Leaves Suitable for Cytoplasmic Genome Amplifying

为了从成熟红麻叶片中提取高质量、高产量的基因组DNA,针对红麻成熟叶片中多糖、多酚含量较高的特性,利用改良CTAB法及改良SDS法分别提取红麻品种福红952成熟叶基因组DNA,并通过琼脂糖凝胶电泳和紫外分光光度计测定进行DNA质量检测。结果表明:改良CTAB法提取的基因组DNA电泳时点样孔干净,条带整齐无拖带,OD260/OD280为1.9左右,产率可达1.84μg/g,其质量、产量都高于改良SDS法,所提取的DNA可用于红麻RAPD分子标记、线粒体DNA、叶绿体DNA通用引物PCR扩增。改良CTAB法是提取成熟红麻叶片DNA的有效方法,并且可用于红麻分子标记及胞质基因组学研究。

The research aimed to extract the high quality and yield genomic DNA from mature kenaf（Hibiscus cannabinus L. ） leaves. According to the character of mature kenaf leaves containing more amylase and polyphenol than young leaves,the kenaf varieties Fuhong 952 mature leaves were extracted by improved CTAB and SDS meth- ods,and they were identified by agarose gel electrophoresis and ultraviolet spectrophotometer methods. The results showed that the sample hole was clean with no pulling, OD260/OD2s0 was about 1.9,the yield ratewas up to 1.84 ~xg/g,and its quality and yield was higher than that extracted by improved SDS method. Genomic DNA extracted by improved CTAB also can be used in kenaf RAPD （Random Amplified Polymorphic DNA） molecular markers,mito- chondrial DNA,and chloroplast DNA universal primer PCR amplification. Therefore, the improved CTAB method was an effective method for extracting the high quality and yield genomic DNA from mature kenaf leaves, and it could be used for the kenaf molecular markers and cytoplasmic genomics research.