鸭肝炎病毒感染和垂直传播的研究

A Study on Natural Infection and Vertical Transmission of DHBV in Ducks

用α-^(32)P-dATP-DHBV DNA探针做斑点杂交试验、检测了不同鸭种血清中携带DHBV情况。其中北京鸭与樱桃谷鸭的杂交种鸭杂交阳性率为5.1％;而不同鸭龄不同饲养条件的高邮麻鸭血清阳性率32.7—58.8％,经EM检测均在血清中找到病毒颗粒。将DHBV DNA不同的亲代配对所组成的四个组分群饲养,观察产卵后再孵化出的雏鸭DHBVDNA阳性率。第一组(♀-(?)-)为0％,第二组(♀+(?)+)为100％,第三组(♀-(?)+)28％,第四组(♀+(?)-)100％。然而亲代雌雄鸭均为DHBV DNA阴性的雌鸭所产卵在孵化到第9天时,经尿囊腔注射1×10~9病毒颗粒/m110μl的血清,孵出雏鸭时DHBV DNA阳性率达83.3％。

DHBV in duck serum is assayed by α-^(32)P-DHBV DNA probe hybridization. It is found that the rate of DHBV viremia in hybrid from Beijing duck and Cherry valley duck was 5.1％, whereas in Gauyou local ducks was32.7-58.8％.Positive sera are confirmed by EM. The virion of DHBV is characterized. Ducks are divided into four groups. The rate of DHBV viremia in tile ducklings hatched from different groups is detected, Group 1 (F^-,M^-) is 0％, Group 2(F^+, M^+)100％, Group 3(F^-, M^+)28％, and Group 4(F^+, M^-)100％. By allantoic route inoculation of DHBV 1× 10~9 virion/ml for 10μl to 9-day old duck embryos from group 1, the rate of DHBV viremia in these hatched ducklings is 83.3％.