BALB/C等乳鼠流行性腹泻(EDIM)的初步研究

Studies on the Epidemic Diarrhea of BALC/c et. Infant Mice

本文研究了1986—1989年间BALB/C小鼠发生的两次散发流行和一次有BALB/C及NIH鼠群暴发流行的EDIM(Epizootic diarrhea of infant mice)。经过电镜、PAGE、实验感染、“ABC”组化抗原定位等证实病原为MRV,而且病毒大小、RNA电泳型与国外报道的MRV有差异。观察了本繁育场内7个品系小鼠,均查出有MRV抗体,但发病只见于BALB/C和NIH鼠群。不同鼠龄和胎次发病率亦有差异。还观察了病变组织的病理组织学,扫描电镜和超微结构的改变。

During 1986-1987, two times of sporadic diarrhea in BALB/c suckling mice and an outbreak of diarrhea in BALB/c and NIH were studied in our breeding farm. The pathogenic agent was identified to be murine rotavirus (MRV) by means of EM, PAGE, infant mice inoculation and 'ABC' antigen location in small intestine. The size of MRV and the RNA migration in PAGE were different from EHP, EW and EB of MRVo There were 7 strains of mice showing positive antibody against MRV without clinic symptom and sign except BALA/c and NIH suckling mice. The EDIM morbidity of mice is dependent upou the strain, age and parity. The changes of pathology, scanning EM and ultrastructure also observed. The transmission of EDIM was discussed in the paper.