洋水仙组培快繁技术研究

Study on techniques of tissue culture and rapid propagation of Narcissus spp.

以洋水仙品种Sun Disc为材料进行组织培养,重点研究洋水仙组织培养中的最佳培养基配方,结果表明:最佳诱导培养基配方为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;最佳增殖培养基配方为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L;最佳促根培养基为1/2MS+NAA 0.5 mg/L,生根率达90%。

Key techniques in tissue culture of Narcissus spp. with focus on optimal prescription were studied using Narcissus var. Sun Disc as raw material. The results showed that, the optimal medium for induction was MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.5 rag/L, for multiplication was MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.2 mg/L, and for induction of roots was 1/2MS ＋ NAA 0.5 mg/L, the rooting rate reached 90%.