云南红药和同类产品对大鼠功血模型子宫内膜修复机制的实验研究

Experimental study of Yunnan hongyao and like product on repair-mechanism of endometrium in dysfunctional uterine bleeding rats

目的:研究云南红药和5个治疗功血的同类产品对子宫出血模型大鼠子宫组织血管内皮生长因子(VEGF)和基质金属蛋白酶-1(MMP-1)含量的影响,探讨该类药物修复子宫内膜的作用机制。方法:采用妊娠大鼠灌胃米非司酮和米索前列醇,造成早孕大鼠不完全流产复制功能性子宫出血模型,观察云南红药对子宫出血模型大鼠的治疗作用,并检测子宫组织中VEGF和MMP-1的含量。结果:与正常对照组大鼠比较,模型组大鼠在造模后,凝血时间明显延长,而云南红药和5个同类产品能明显缩短子宫出血模型大鼠的凝血时间,与模型组相比有统计学差异(P<0.01)。与正常对照组大鼠比较,模型组大鼠在造模后,子宫组织的VEGF含量明显降低,而MMP-1的含量明显升高(P<0.01)。云南红药+戊酸雌二醇片组能明显降低子宫出血模型大鼠子宫组织MMP-1的含量,与模型组相比有统计学差异(P<0.05),而云南红药胶囊组、戊酸雌二醇片组、宫血宁胶囊组、云南白药胶囊组、茜芷胶囊组及五加生化胶囊组对子宫组织MMP-1的含量没有明显影响(P>0.05)。云南红药胶囊组、云南红药+戊酸雌二醇片组、宫血宁胶囊组及五加生化胶囊组能明显增加子宫出血模型大鼠子宫组织VEGF的含量,与模型组相比有统计学差异(P<0.01或P<0.05),而戊酸雌二醇片组、云南白药胶囊组及茜芷胶囊组对子宫组织VEGF的含量没有明显影响(P>0.05)。结论:云南红药和5个同类产品对功能性子宫出血有明显的治疗作用,其中云南红药胶囊、宫血宁胶囊及五加生化胶囊对子宫内膜有明显的修复作用,其作用机制可能与上调VEGF有关;云南红药胶囊和戊酸雌二醇片合用可以增强其修复子宫内膜的作用。

AIM. To study the effect of Yun- nan hongyao and other 5 like products on VEGF and MMP-1 levels of uterine tissue in dysfunc- tional uterine bleeding （DUB） rats. And explore the repair-mechanism of endometrium of thesedrugs. METHODS： The models of DUB were established by administering intragastrically with mifepristone and misoprostol to pregnant rats. After management, the clotting time were observed, and the VEGF and MMP-1 levels of uterine tissue were determined with ELISA method. RESULTS： Compared with the model group, the clotting time were significantly de- creased （P ~ 0.01） in Yunnan hongyao and 5 like products groups. In addition, the level of VEGF of uterine tissue was significantly de- creased and the level of MMP-1 was significantly increased in model group. Compared with the model group, the levels of VEGF were signifi- cantly increased in Yunnan hongyao,Gong xuen- ing and Wujia shenghua groups （P 〈 0.01 or 0.05）, and the level of MMP-1 was significantly decreased in Yunnan hongyao＋estradiol valerate group （ P 〈 0.05 ）. CONCLUSION. Yunnan hongyao and 5 like products have significant therapeutic effect on DUB rats. Yunnan hongyao,Gong xuening and Wujia shenghua can repair endometrium. The mechanism is relevant to increasing the levels of VEGF.