人脊髓胚胎血管发育的解剖学研究

Anatomic Study on Embryonic Development of Human Fetal Spinal Vascular System

目的:探讨人脊髓内胚胎血管发育的形成方式。方法:合法获取胎龄35 d～43 d的人胚胎,采用HE、Nissl染色观察脊髓内血管形成方式和过程。结果:胚胎35 d时,脊髓周围的间充质内已出现了毛细血管网,形成髓周动脉冠,脊髓实质内还没有血管生成。胚胎43 d时,脊髓实质内以成血管生成方式形成血管索与表面的毛细血管垂直向脊髓内发出分支进行对接并在髓内发出分支,构成毛细血管网。结论:人胚胎脊髓内血管生成可能主要以成血管生成的方式形成实质内血管索,与髓周血管进行对接形成髓内毛细血管网。与胚胎脑的血管生成方式不同。

Objective ： To describe the topography of and the cellular processes that underlie vascularization of the buman fetal spinal cord. Methods： The vasculature of human spinal cord obtained from fetuses ranging in age from 35 to 43 days are examined in HE,Nissl stained, and by anti CD34 immunohistochemistry. Results： The first event in spinal cord vascularization was the migration of large numbers of spindle shaped mesenchyme precur sor ceils, these cells proliferated and differentiated to produce cords of endothelial cells and form the primary vessls. These processes are consistent with vessel formation by vasculogenesis. Conclusion： These observations sug gest that the formation of primordial vessels in the spinal cord is mediated by vasculogenesis, whereas angiogenesis is responsible for increasing vascular densitY. These mechanisms of spinal cord vascularization appear contrast to those of vascularization of the brain during development.