应用免疫电镜技术快速检测菜豆黄花叶病毒、马铃薯M病毒和燕麦花叶病毒

Rapid Detection of Bean Yellow Mosaic Virus, Potato Virus M and Oat Mosaic Virus Using Immunosorbent Electronmicroscopy

应用免疫吸附电流技术(ISEM)可有效地检测腐汁液中的菜豆黄花叶病毒(BYMV)、马铃薯M病毒(PVM)和燕麦花叶病毒(OMV)。BYMV,PVM和OMV三种抗血清的适宜工作浓度和对铜网的适宜包被时间均为1000倍和1小时,对同源病毒的适宜捕获时间分别为4℃下2、2和8小时。PVM和OMV的病汁液检测灵敏度均为稀释4000倍,而BYMV病汁液稀释16000倍时还能检测到少量病毒料子。ISEM捕获法和修饰法的结果表明,这三种病毒之间无血清学交叉反应。

Bean yellow mosaic virus (BYMV), potato virus M(PVM) and oat mosaic virus (OMV)were rapidly detected in infected leaf extracts using immunosorbent electron microscopy. The suitable dilution and time for coating the three antisera in carbon coated grids were 1000 and 1h at room temperature. The suitable trapping time for BYMV, PVM and OMV were 2,2 and 8h respectively at 4℃. The detective sensitivities in leaf saps were 4000 dilutions for PVM and OMV, and a few virus particles could still be seen at the dilution of 16000 of BYMV infected leaf sap. No cross reaction happened among these 3 viruses by trapping and decorating tests.