摘要
油桐尺蠖核型多角体病毒(BsNPV)自1978年分离以来,对其形态结构、形态发生、毒力测定、血清学、理化特性、安全性试验、剂型研究及大田应用等已进行了广泛的研究,其基因组物理图谱也已构建。采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)研究BsNPV蛋白质时发现,多角体蛋白与病毒粒子的一种主要蛋白质的分子量非常接近,
Aspartyl-Prolyl bonds in proteins could be selectively hydrolyzed with 75％ formic aeid. The in situ aeidie cleavage procedure based on acid lability of Asp-Pro bonds is very important in sequence analysis of proteins and comparative studies of related or similar proteins, Limited cleavage of polyhedrin and virion 31k polypeptide which have the same molecalar weights in SDS-PAGE of Buzura suppresaria nuclear polyhedrosis virus by above method produced 4 and 8 bands, respectively. The results suggest that the two proteins are different The number of Asp-Pro bend in these two proteins is also discussed.
关键词
油桐尺蠖病毒
原位化学切割
BsNPV
In silu chemical cleavage Asp-Pro peptide bond Buzura suppressaria nuclear polyhedrosis virus (BsNPV) 31k protein
