介体蚜虫个体带毒(BYDV)免疫电泳测试技术初步研究

Study on Technique of Immune Electrophoresis of BYDV Carrying Vector Aphids

经对小麦黄矮病(BYDV)介体蚜虫同一带毒个体,进行一苗一虫接种传毒后,用免疫电泳双重测试,室内人工饲毒蚜虫接种传毒发病与免疫电泳阳性反应完全一致,由接种传毒发病试验证明免疫电泳测试介体蚜虫个体带毒具有可行性。这是介体蚜虫传播小麦黄矮病个体带毒快速检测技术首次获得成功。

Virus transmission was conducted through inoculation of aphids from the same virus carrying individuals with BYDV which was one insect per seeding. The result showed that the duplex immune eleetrophoresis showed positive reaction coinciding with the disease incidence induced by aphids fed with virus artificially at the laboratory, Through the experiment of inoculation and transmission, it was manifested that it was possible to test the virus carrying potential of the vector aphids through immune electrophoresis method This is the first report in the field.