棉花区试品种的AFLP指纹图谱分析

AFLP Fingerprinting Analysis of 31 Cultivars Attending the National Regional Trial

为了探索AFLP分子标记在构建国家区试棉花品种指纹图谱上的优势,以2009年和2010年参加国家黄河流域棉花区试的B组常规棉、C组杂交棉共31份品种为材料,运用筛选确定的2对AFLP核心引物对这些品种进行分子标记分析,共获得44个多态性片段,其中多态性位点占总扩增位点的28.39%,利用44个多态性片段初步构建了这些品种的AFLP指纹图谱。聚类结果显示,在相似系数为0.92时,31份区试品种完全区分开;常规棉之间、杂交棉之间大多不同程度的优先聚集在一起。

For exploring the advantage of AFLP molecular marker in constructing the cotton fingerprinting, 31 cultivars of conventional cottons in team B, hybrid cottons in team C attending the 2009 and 2010 national regional trials were used for DNA fingerprinting. Two pairs of core AFLP primer were used for polymorphism analysis. Forty-four polymorphic bands were obtained which took 28.39% of total amplified bands. A primary DNA fingerprint database was established with 44 polymorphic bands. The cluster analysis showed that, all tested-cultivars could be completely identified at 0.92 similarity index level, and conventional cottons almost cluster together preferentially in different extent, so as hybrid cottons.