耐旱转录因子CBF3水稻育种应用研究初报

Preliminary Report on the Study of the Application of Transcription Factor CBF3 to Rice Breeding

转基因结果表明,DREB/CBF转录因子家族在植物抗逆品种改良中具有重要的应用价值。利用常规杂交育种技术将CBF3基因转入籼稻强优势恢复系,并对其转入后在干旱条件下对水稻亲本恢复系农艺性状的影响进行研究。结果表明:转基因3(含CBF3基因)和其对照转基因5(不含CBF3基因)亲本间干旱条件下农艺性状差异不大。而转基因3、对照转基因5与恢复系杂交F2干旱条件下农艺性状变化则差异较大,其中,在9311等部分材料上表现为株高明显增加,每穗总粒数、每穗实粒数、千粒重、单株谷重等明显降低,不适合配组,而另一部分材料如OP193等在有效穗、千粒重、单株谷重等方面则表现有较好的配合力。该项研究为进一步进行含该基因的具有显著耐旱性能的籼稻杂交稻新品种育种奠定材料、方法基础。

Transgenic results showed that DREB/CBF transcription factors had important application value for improvement of plant stress-resistant breeding. In this study, by using of crossbreeding, CBF3 was transferred into rice indica strong restorer line, and the effect of CBF3 on the agronomic characters of the progenies of F2 restorer line under drought condition were studied. The results indicated that, the agronomic characters of transgenic rice 3 with CBF3 and nontransgenic rice 5 had little difference under drought condition, but in the cross between transgenic rice 3, nontransgenic rice 5 and indica strong restorer line, the agronomic characters of F2 made a great difference under drought condition, parts materials, such as 9311 etc., were manifested as that plant height increased significantly, the grain number per panicle, filled grain number per panicle, 1000-grain weight and grain weight per plant etc. decreased significantly, but another parts, such as OP193 etc., showed with fine combining ability in the number of effective panicle, 1000-grain weight, grain weight per plant etc. The study provided a reference for the further research in the new indica hybrid rice varieties breeding, which was with CBF3, and showed as super drought tolerance.