啤酒花不同外植体再生体系的研究

The Study on Different Explant of Hops Regeneration System

为了建立适用于基因工程培育抗病毒的啤酒花再生体系,筛选了‘于勒比特’啤酒花(Humuluslupulus L.)不同外植体、不同培养基、不同激素配比对愈伤组织形成、不定芽分化和不定根产生的影响。结果表明:叶盘、节间和腋芽都可以诱导形成愈伤组织,且叶盘在MS-B5培养基+葡萄糖20 g/L,含有6-BA 0.5 mg/L+IAA 1.5 mg/L,以7 g/L琼脂固化,pH 5.8的条件下,愈伤组织诱导率可达92.5%,愈伤组织形成不定芽可达56.0%,初步构建了‘于勒比特’啤酒花的再生体系。

The regeneration systems from leaf, axillary bud and internodes explants of ＇Yulebite hops＇ （Humulus lupulus L.） were established in order to culture resistant cultivar by gene engineering technology. The result showed that the leaf, axillary bud and internodes from in vitro cultures of hops are able to regenerated shoots by direct organogenesis. But the leaf was better for establishing than others. The highest regeneration of the leaves （92.5%） and differentiation （56.0%） of callus was achieved from the MS-B5 mediums with sucrose 20 g/L, IAA 1.5 mg/L and 6-BA 0.5 rag/L, agar 7 g/L, pH 5.8, respectively.