一株辣椒花叶病毒的生化特性研究

Study on Biochemical Characteristics of a Capsicum Mosaic Virus

本文对辣椒花叶病毒的生化特性进行了研究。病毒蛋白的紫外吸收,最高280毫微米,最低为260毫微米;以SDS-PAGE测定其分子量为17500;病毒蛋白亚基由148个氨基酸组成。从病毒提纯的核酸为线状RNA;紫外吸收最高为260毫微米,最低230毫微米;经聚丙烯酰胺凝胶电泳测定,其分子量为2.0×10~6。根据形态结构、血清学试验、外壳蛋白亚基组成、核酸和蛋白的分子量等综合分析,我们认为这株辣椒花叶病毒是烟草花叶病毒组(TMV)中的一个毒株。

A Capsicum Mosaic Virus(CaMV) was studied on some biochemical charaeteristics. UV-absorbance curve of viral protein at 280nm is maximum, at 260nm is minimum. The molecular weight of coat protein is 17500, consisting of 148 amino acid residues. The purified nucleic acid is confirmed as linear RNA, UV absorbance of virus RNA at 260nm is maximum, at 230nm is minimum. Polyacryamide gel electrophoresis of viral RNA showed that molecular weight is about 2×10~6. On the basis of the morphologieal structure of the virion, serological test, amino acid composition of coat protein snbunits and molecular weight of virus RNA and protein, we preliminarily conclude that the CaMV is a strain of Tobocco Mosaic Virus.