摘要
以感染肾综合征出血热病毒(HFRSV)的Vero E6细胞为材料,用免疫亲和层析结合制备聚丙烯酰胺凝胶电泳(PAGE)从感染细胞中提纯了HFRSV两种糖蛋白。先用免疫亲和层析从感染细胞的粗制抗原中获得含有四种蛋白的混合液,用[~3H]-氨基葡萄糖在感染细胞中标记病毒糖蛋白,观察到[~3H]-氨基葡萄糖只结合入78K和57K的病毒蛋白。再用制备SDS-PAGE从HFRSV混合液中提纯78K和57K两种蛋白。实验证明这两种糖蛋白均具中和抗原决定簇,57K的糖蛋白尚具血凝活性,初步鉴定表明这两种糖蛋白相当于文献报道的HFRSV G_1和G_2。
Two HFRS virus glycoproteins with molecular weight of 78K and 57K were purified by immunoaffinity chromatography with preparative SDS-PAGE from virus-infected cells Purified 78K and 57K glycoproteins all showed only one band on SDS-PAGE and have antigenieity and immunogenicity, The results of hemagglutination test indicate that 57K glyeoprotein has hemagglutation activity. Neutralizing test with antisera confirm both 78K and 57K glyeoproteins have neutralizing antigenic determinants. These results suggest that these two glycoproteins are analogous to G1 and G2 glycoproteins of virion of HFRS virus.
出处
《病毒学杂志》
CSCD
1991年第2期143-146,共4页
基金
国家自然科学基金
关键词
HFRSV
糖蛋白
提纯
鉴定
Virus of hemorrhagic fever with renal syndrome Glycoprotein Affinity chromatography
