流行性出血热病毒体外直接激活补体C_3的研究

Study of EHFV Directly Activating Complement C3 in Vitro

用蔗糖梯度纯化的4株不同来源的流行性出血热(EHF)病毒(陈株、H8205、ALC96、R22)抗原,在体外直接与健康人血清于37℃作用30分钟,用交叉免疫电泳检测血清中C_3激活情况。结果陈株、H8205、R22 3株病毒抗原,可以直接激活补体C_3,电泳后呈现典型降解峰,而ALC96与正常细胞抗原则均为阴性。病毒抗原激活C_3的活性,与病毒核衣壳蛋白(Nucleocapsid Protein)的含量及毒株对乳鼠的半数致死量有关,并可被特异性抗体(IgG)所中和。

The antigens of four EHFV strains from different sources(Chen, H8205, ALC96, R22)purified by sucrose gradient centrifugation incubated with normal human sera in 37℃ for 30 minutes Cross-immunoelectrophoresis was used to detect whether complement C_3 was activated. The results showed that Chen, H8205, R22 strains antigens can directly activate C_3, typical degradative peak was found after electrophoresis. But ALC96 and normal antigen were negative. Ability of activating C_3 of EHFV antigens was related to the content of EHFV nucleocapsid protein and LD50 of virus strain to suckling mice, which was neutralized by specific antibody (IgG).