3T3成纤维细胞和巨噬细胞在含rhbFGF培养基血纤维上的共培养

Co-culture 3T3 fibroblast and macrophage on blood fibrin clot with rhbFGF

目的 :了解 3T3成纤维细胞和巨噬细胞在含重组人碱性成纤维细胞生长因子 (rhbFGF)培养基血纤维上的生长行为 .方法 :采用四甲基偶氮唑盐 (MTT)比色法、相差显微镜观察、吉姆萨染色和扫描电子显微镜观察方法 .结果 :在低血清含rhbFGF的培养条件下 ,3T3成纤维细胞和巨噬细胞在血纤维上能够良好生长 .结论 :本研究揭示 ,血纤维不仅可以作为一种低免疫原性或无抗原性的生物支持材料用于三维组织培养 。

Aim: To investigate the behaviour of 3T3 fibroblast and macrophage co-culture on blood fibrin clot with recombinant basic fibroblast growth factor (rhbFGF). Methods: Using MTT method, inverted contrast microscopy, Giemsa staining as well as scanning electron microscope. Results: the effect of rhbFGF on co-culture of 3T3 fibroblast and mouse macrophage on blood fibrin clot in low-serum DMEM with rhbFGF were monitored, and 3T3 fibroblast and macrophage growed well on the blood fibrin blot in low-serum DMEM with rhbFGF. Conclusion: The blood fibrin clot, a low immunogenicity or non-antigen, could be used as a bionic support for three-dimensional tissue culture, and also a physiological carrier to distribute the growth factor rhbFGF for the cells. [