黄芪对肝星状细胞c-fos和c-jun基因表达的调节

Regulation of astragalus on c-fos and c-jun gene expression of hepatic stellate cells

目的观察黄芪对肝星状细胞c—fos和c-jun基因表达的影响。方法在培养的肝星状细胞株中分别加入不同浓度的黄芪（终质量浓度1．0、0．5g/L）；于8、24、48、72h4个时间点分别收集细胞，提取肝星状细胞总RNA；用逆转录定量聚合酶链反应（RT．qPcR）测定c．fos和c_jun的基因表达水平。结果加入黄芪的两组HsC—T细胞c—fos基因表达水平在8h（0．32±0．10、0．50±0．12）、24h（0．24±O．08、O．42±0．10。）、48h（O．20±0．04、0．34±0．07）、72h（0．12±0．03、0．23±0．05）4个时间点均明显低于对照组（0．63±0．13、0．50±0．12、0．43±0．06、0．32±0．04）；c-jun基因表达水平在8h（O．46±O．10、O．66±O．12）、24h（O．36±0．08、O．57±O．11）、48h（O．27±O．08、0．43±O．10）、72h（0．19±0．03、0．32±0．05）4个时间点也均明显低于对照组（0．93±0．13、0．80±0．12、0．63±0．10、0．46±0．07）。随剂量加大，Hsc—T细胞c怕s、cIjun基因表达水平逐渐降低；两组间差异有统计学意义（P〈0．05）。结论黄芪对肝星状细胞c．fos和c_jun基因表达有明显下调作用。

Objedive To investigate the effects of astragalus the on c-fos and c-jun gene expres- sion in hepatic stellate cells. Methods .Hepatic stellate cells （HSC-T6） were cultured in medium contai- ning different concentrations of astragalus （1.0, and O. 5 g/L） and harvested at different incubation time points （8, 24, 48, and 72 h）. Total RNA of HSC-T6 cells was isolated and the c-fos and c-jun gene ex- pression levels were detected by using reverse transcription polymerase chain reaction. Results The c-fos gene expression levels of HSC-T6 ceils in two astragalus groups were significantly lower than those in con- trol group （0. 63±0. 13, 0. 50±0. 12, 0. 43±0. 06, and 0. 32±0. 04） after exposure to astragalus at 8 h （0.32±0. 10, 0.50±0. 12）, 24h （0.24±0.08, 0.42±0. 10）, 48h （0.20±0.04, 0.34±0.07） and 72 h （0. 12±0. 03, 0. 23±0.05 ）. The crjun gene expression levels of HSC-T6 cells in two astragalus groups were also significantly lower than those in control group （0. 93±0. 13, 0. 80±0. 12, 0. 63±0. 10, and O. 46±0. 07） after exposure to astragalus at 8 h （0. 46±0. 10）： 66±0. 12）, 24 h （0. 36 ＋0. 08, 0.57±0.11）,48h （0.27±0.08, 0.43-＋0.10） and72h （0.19±0.03, 0.2±0.05）. The c-fos and c-jun gene expression levels in HSC-T6 cells were decreased gradually as the dosage of astragalus in- creased, and there were significant differences in the c-los and c-jun gene expression between two astraga- lus groups. Conclusion Astragalus may down-regulate the c-fos and e-jun gene expression in hepatic stel- late cells.