树突状细胞杂交瘤苗诱导免疫效应细胞对PC3生长的抑制作用

Inhibitory effect of mmunoeffector cells on growth of PC3 induced by dendritic cell fusion hybrids

目的观察胰腺癌细胞PC3-树突状细胞（DC）杂交瘤苗诱导免疫效应细胞对PC3的抑制作用。方法将DC与胰腺癌PC3细胞融合制备PC3一DC融合细胞（简称DC瘤苗），磁式分选器分选DC瘤苗；酶法检测DC瘤苗诱导免疫效应细胞对PC3的毒性作用；分别用18只裸鼠进行DC瘤苗体内致瘤实验及DC瘤苗诱导免疫效应细胞对PC3移植瘤生长抑制效果实验。结果PC3．DC杂交瘤苗诱导免疫效应细胞对PC3的杀伤效率最高，其杀伤效率高达76．6％。裸鼠实验发现阴性对照组（I）。、细胞治疗组（Ⅲ．）均于第12天发生移植瘤；预防治疗组（Ⅱ）观察30d时，6例只有1例发生移植瘤；于移植瘤产生后的第45天处死所有裸鼠并称瘤体的重量，各组比较差异有统计学意义（P〈0．05），组1分别与组Ⅱ、Ⅲ比较差异有统计学意义（P〈0．001），组Ⅱ与组Ⅲ比较差异有统计学意义（P〈0．05）。结论DC瘤苗能有效诱导免疫效应细胞抑制恶性肿瘤生长。

Objective To observe the inhibition of immunoeffector cells on the. growth PC3 cells induced by dendritic cell fusion hybrids. Methods DCs were fused with PC3 cells and fusion cells （DC fusion hybrids） were marked with CDi 1 c MicroBeads. In vitro, cytotoxin study, the immunoeffector cells were divided into A1 group （without DC stimulated）, A2 group （DC stimulated）, A3 group （DC fusion hybrids stimulated）. During the in vivo study, 18 nude mice were allocated to 3 groups; preventive group （ II ） , 1-2 days before inoculating PC3, giving DC fusion hybrids activated immunoeffector cells; treated group （~） , giving DC fusion hybrids activated immunoeffeetor cells after emergence of implanted tumor in all nude mice; control group （ I ） , giving equivalent amount of 1640 cultured liquid. Results In vitro, the maximal killing rate was A3 group （76. 6% ）. /n vivo, 12 mice developed implanted tumor on the day 12 in group I and m, separately; In group II , on the 30th day, 1 of the 6 mice developed implanted tumor. On the 45th day after development of implanted tumor, all the nude mice were. sacrificed and the tumor body weighed. There was significant difference among group I ,Ⅱ, Ⅲ （P 〈 0. 05 ）. Conehlsion DC fusion hybrids may piay an important role in anti-tumor therapy.