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二烯丙基三硫化物对人神经母细胞瘤细胞SK—N—SH生长的抑制作用 被引量:2

Experimental study on inhibition the growth of neuroblastoma by diallyl trisulfide in vitro
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摘要 目的探讨二烯丙基三硫化物(DATS)对人神经母细胞瘤细胞SK.N.SH生长的影响及相关机制。方法光镜下观察不同浓度DATS对SK—N—SH细胞生长的影响;细胞分为对照组、DATSI组(10p晷/L)和DATSⅡ组(40肛∥L),DATS作用24、48h后,流式细胞仪测定细胞凋亡;作用72h后,荧光定量聚合酶链反应(FQ—PCR)法测定DATS对SK—N—SH细胞血管内皮生长因子(VEGF)mRNA、细胞间黏附分子一1(ICAM.1)mRNA表达的影响;酶联免疫吸附试验(ELISA)法测定细胞培养上清液VEGF、ICAM.1浓度。结果不同浓度的DATS均能抑制SK—N—SH细胞的生长,小剂量DATS能诱导细胞凋亡;对照组、DATSI组和Ⅱ组VEGFmRNA、ICAM一1mRNA表达量分别为5.974-1.31、4.21±1.06、3.35±0.77(P〈0.05)和4.41±O.94、3.22±1.17、2.69±O.73(P〈0.05);对照组、DATSI组和Ⅱ组SK—N.SH细胞培养上清液中VEGF、ICAM一1分别为(72.14-26.9)、(51.6±17.3)、(40.3±11.8)μg/L(P〈0.05)和(311.6±83.7)、(274.2±71.0)、200.6±42.9)ng/L(P〈0.05)。结论DATS在体外可抑制神经母细胞瘤细胞SK-N—SH的生长,DATS能诱导SK—N-SH细胞凋亡,下调其VEGFmRNA、ICAM一1mRNA的转录和蛋白表达。 Objective To investigate the efficacy and the possible mechanism of human neuroblas- toma cell line SK-N-SH induced by diallyl trisulfide (DATS) in vitro. Methods Flow cytometry was ap- plyed to test the apoptosis rate of human neuroblastoma cell lines SK-N-SH after DATS was used 24 h and 48 h. SK-N-SH cells were randomly divided into 3 groups: the control group, the DATS group I (DATS 10 trg/L) and group lI ( DATS 40 μg/L), the role of DATS on the growth of SK-N-SH ceils was evalua- ted after 72 h. The expression of vascular endothelial growth factor (VEGF) mRNA and intercellular adhe- sion molecule-1 ( ICAM-1 ) mRNA of SK-N-SH ceils were detected by fluorescent quantitation ploymerase chain reaction (FQ-PCR). The VEGF and ICAM-1 concentration of supernatants were measured by enzyme linked immunosorbent assay (ELISA). Results DATS inhibited the growth of SK-N-SH cells in vitro, small dose of DATS induced apoptosis of SK-N-SH cells (P 〈 O. 05 ). The expression of VEGF mRNA in the control group, the DATS group I and DAIS group II was (5.97 ± 1.31 ), (4. 21 ± 1.06) and (3.35±0.77) (P〈0.05) , the expression of ICAM-1 mRNA was (4.41 ±0.94) , (3.22 ±1.17) and (2.69 + 0. 73) (P 〈 0. 05 ). The VEGF concentration of culture supernatants of SK-N-SH cells was ( 72. 1 ± 26. 9), (5L 6 ± 17. 3) and (40. 3 ± 11.8) μg,/L in the 3 groups (P 〈0. 05), and the ICAM-1 concen- tration was (311.6 ± 83.7 ), ( 274. 2 ± 71.0 ) and ( 200. 6 + 42. 9 ) ng/L ( P 〈 0, 05 ). Conclusion DAIS can inhibit the growth of human neuroblastoma cell lines SK-N-SH in vitro, which may caused by in- ducing apoptosis and down-regulating the expression of VEGF and ICAM-1.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2013年第3期533-535,共3页 Chinese Journal of Experimental Surgery
基金 广东省医学科研基金资助项目(A2012509)
关键词 二烯丙基三硫化物 肿瘤 神经母细胞瘤 血管内皮生长因子 细胞间黏附分子 Diallyl tsulfide Neoplasm Neurobtastoma Vascular endothelial growth factor Intercellular adhesion molecule
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