星形细胞上调基因-1和血管内皮生长因子在胆管癌组织中的表达及其临床意义

Expression and clinical significance of astrocyte elevated gene-1 and vascular endothelial growth fac-tor in cholangiocarcinoma

目的探讨星形细胞上调基因．1（AEG-1）和血管内皮生长因子（VEGF）在胆管癌中的表达水平及临床意义。方法用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（sP）法检测112例胆管癌组织和29例癌旁正常组织中AEG-1和VEGF的表达水平。结果AEG-1和VEGF在胆管癌中的表达阳性率分别为71．4％和70．5％，高于癌旁正常组织0％（P〈0．01）。AEG-1在胆管癌中的表达阳性率与组织学分级（高分化45．5％、中分化64．9％、低分化86．8％）、临床分期（I～Ⅱ期61．8％、HI—IVa期91．7％）、淋巴结转移（无淋巴结转移61．8％，伴淋巴结转移91．7％）呈正相关（P〈0．05），而与患者年龄和病理类型无明显相关（P〉0．05）。结论胆管癌中AEG-1和VEGF高表达提示预后较差，可作为临床评估预后的指标。

Objective Toinvestigate the expression and clinical significance of astrocyte elevated gene-1 （ AEG-I ） and vascular endothelial growth factor （VEGF） in eholangiocarcinoma. Methods The expression levels of AEG-1 and VEGF in bile duct neoplasms were detected by using immunohistochemis- try, including 112 cases of malignant tumors and 29 cases of benign tissues. Results The positive expres- sion rate of AEG-1 and VEGF was respectively 71.4% and 70. 5% in malignant tumors, higher than 0% in benign tissues （P 〈0. 01 ）. The positive expression rate of AEG-1 was positively associated with histo- logical classification （well-differentiated 45.5%, moderately differentiated 64. 9%, and poorly differentia- ted 86. 8% ）, clinical stage （ stage I -Ⅱ 61.8%, stage Ⅲ-Ⅳ a 91.7% ） and lymph node metastasis （ positive lymph node metastasis 61.8%, and negative lymph node metastasis 91.7% ） （ P 〈 0.05 ）, but not with ages and pathologic types （ P 〉 0. 05 ）. Conclusion Overexpression of AEG-1 and VEGF in cholangiocarcinoma suggests poor prognosis. They can be used as detection indexes to evaluate prognosis.