改良型大鼠梗阻性黄疸肝切除术后肝衰模型的建立

Rat model of postoperative liver failure induced by modified method of hepatectomy with obstructive jaundice

目的建立一种改良的Wistar大鼠梗阻性黄疸肝切除术后肝衰竭模型。方法通过切断胆总管建立梗阻性黄疸模型，探索最佳梗阻时间。在梗阻14d后，行胆道内引流，分离出70％肝脏分别阻断0、15、30、45min，切除未行阻断的余肝，建立肝衰模型，观察生化指标、病理改变及生存率。结果梗阻14d组更加适合下一步造模。随着阻断时间的延长肝功能进行性下降，各组间差异有统计学意义（P〈0．05），其中30min组24h死亡率达80％，有典型肝衰症状，组织病理学符合肝衰特点。结论对梗阻性黄疸大鼠行肝脏血流阻断及肝切除可建立能较大程度模拟临床过程的外科型肝衰竭模型。

Objective To establish a modified operative method-induced postoperative liver failure in Wistar rats. Methods An obstructive jaundice model with bile duct cut off in rats was established to study the optimum obstruction time. Besides, a rat model of liver failure was established. After obstruction for 14 days, the rats were subjected to internal biliary drainage. Afterwards, the vessel of the left and mid- die lobe of liver （occupied 70% of the total volume） for different time, then resected the other lobes of liver without ischemia. To observe and compare the biochemical indexes, the histopathology, and the survival time. Results 14 d group is more suitable for the next treatment. The liver function of the rats were de- creased with prolonging the time of ischemia, the differences between groups were significant （ P 〈 0. 05 ）. The mortality was as high as 80% after 24 hours in 30 min group, and this group had the typical symptoms of liver failure and the pathology was significant Conclusion The experimental model of postoperative liver failure could be established by partially hepatectomized after hepatic devascularization in Wistar rats with obstructive jaundice. It showed similar clinical pathophvsiological changes in hunman beings.