Wnt信号通路调控因子Pygo2在人肾癌组织中的表达及意义

Expression and the clinical significance of Wnt signal pathway regulation factor Pygo2 in human renal cell carcinoma

目的研究Wnt信号通路调控因子Pygo2在。肾癌组织中的表达及意义。方法经病理检查确诊为。肾透明细胞癌组织标本42例。肿瘤直径1．2—15．5cm，平均7．2cm；其中〈4．0cm7例，4．0～7．0cm9例，〉7．0cm26例。Fuhrman分级：Ⅰ级6例，Ⅱ级17例，Ⅲ级17例，Ⅳ级2例。AJCCTNM分期：Ⅰ期16例，Ⅱ期7例，Ⅲ期7例，Ⅳ期12例。采用RFQ—PCR及免疫组织化学sP法检测Pygo2在肾癌组织及癌旁正常肾脏组织中的表达。统计学分析癌旁正常肾组织与肾癌组织、肾癌组织各组内的表达差异情况。结果肾癌组织与癌旁组织中Pygo2mRNA表达量分别为2．88±1．26及1．00±0．00，蛋白表达量分别为45．53±24．54及11．02±1．39，组间差异均有统计学意义（P〈0．0001）。肾癌组织中Pygo2表达与病理分级、临床分期密切相关，特别是与有无淋巴结转移密切相关（P〈0．0001），而与性别、年龄无关（P〉0．05）。结论Pyg02在病理高分级、临床高分期、发生淋巴结转移的肾癌组织中高表达，提示可能在肾癌发生发展过程中具有重要作用。

Objective To study the expression and the clinical significance of Wnt signal pathway regulation factor Pygo2 in human renal cell carcinoma. Methods Using RFQ-PCR and immunohistochem- ical SP methods to detect Pygo2 mRNA and protein expression in 42 cases renal clear cell carcinoma and their own normal kidney tissue specimens. All specimens had a definite diagnosis by pathologic. All were renal clear cell carcinoma. The tumor diameter was 1.2 - 15.5 cm. The average was 7.2 cm. Among all patients, there were 7 cases with diameter 〈4.0 cm, 9 cases 4.0 - 7.0 cm, 26 cases 〉 7.0 cm. Fuhrman classification： grade I 6 cases, grade II 17 cases, grade III 17 cases, grade IV 2 cases. AJCC TNM stages： stage 1 16 cases, stage II 7 cases, stage III 7 cases, stage IV 12 cases. Statistics was done to analyze the ex- pression difference of pygo2 between normal kidney and renal cell carcinoma, and among renal cell carcinoma within each group. Results There was higher expression of Pygo2 in renal cell carcinoma, and in the adjacent lower expression. The pygo2 mRNA expression were 2.88 ± 1.26 and 1.00 ± 0.00 in respective specimens （P 〈 0. 0001 ）. The pygo2 protein expression were 45.53 ± 24.54 and 11.02 ± 1.39 in respective specimens （ P 〈 0. 0001 ）. Pygo2 expression in grading and staging were statistically significant （ P 〈 0. 0001 ）, but in gender, age was not statistically significant （P 〉 0.05）. Conclusions High expression of Pygo2 was found in Fuhrman high grade, high clinical staging, lympho-metastasized renal clear cell carcinoma. Pygo2 might play an important role in the occurrence and development process in renal clear cell carclnoma.