高钾溶液对慢性非细菌性前列腺炎SD大鼠前列腺平滑肌细胞内钙离子浓度的影响及意义

Change of intracellular calcium concentration in prostate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution

目的探讨慢性非细菌性前列腺炎sD大鼠前列腺平滑肌细胞（PSMC）内游离钙离子浓度（[Ca2＋]i）在高钾溶液作用下的变化及临床意义。方法SD大鼠40只，随机分为实验组和对照组，采用去势＋雌二醇注射的方法诱导大鼠非细菌性前列腺炎，体外培养纯化PSMC，细胞经钙离子荧光探针QuestFluo-8孵育后在激光共聚焦显微镜下连续动态扫描，然后加入高钾溶液250ul（60mmol／L），观测细胞内钙离子荧光强度变化情况。结果光镜下实验组标本显示典型慢性前列腺炎的病理特征，对照组未见炎症表现。免疫细胞化学方法证实两组细胞均为PSMC。两组PSMC内钙离子荧光强度增加幅度分别为27．86±9．88和7．61±4．31，组间差异有统计学意义（P〈0．01）。结论高钾溶液导致细胞内钙离子浓度升高，可能会影响细胞及前列腺的各项电生理功能，在慢性前列腺炎发病机制中可能具有一定作用。

Objective To investigate the change of intracellular calcium ion concentration in pros- tate smooth muscle cells of SD rats with chronic abacterial prostatitis under high potassium solution. Methods SD rats were divided into experiment group and control group. The CP model was set up by castration and estradiol injection. The PSMC was cultured and purified in vitro. Laser confocal scanning microscope was used after the ceils were incubated with Quest Fluo-8TM. The cells were treated with high potassium solution, and the change of fluorescence intensity was observed. Results The pathologic specimens of the experiment group showed typical pathologic characteristics of chronic prostatitis under light microscope, the control group without inflammation performance. Using immunocytochemistry method confirmed that the experiment group and the control group were prostate smooth muscle cells. The change of fluorescence intensity of [ Ca2＋] i in the experiment group and control group in the high potassium solution was 27.86 ± 9.88 and 7.61± 4.31. There were statistically significant differences between the two groups （ P 〈 0.01 ）. Conclusions High potassium solution cause intracellular calcium ion concentration increased.