金银花愈伤组织的诱导及继代培养研究

Study on Callus Induction and Subculture of Lonicera japonica Thunb.

以金银花优良品种"金丰一号"为试材,研究了不同外植体及不同浓度激素组合对金银花愈伤组织诱导和继代的影响。结果表明:愈伤组织诱导时,最佳外植体为花蕾下部,最佳培养基为MS+NAA 2.0mg/L+6-BA 0.01mg/L+2,4-D 0.5mg/L,诱导率可达100%,且愈伤组织生长较快,量多,质地疏松、湿润,颜色鲜亮;愈伤组织继代时,最佳培养基为MS+6-BA 2.0mg/L+KT 0.75mg/L+NAA 0.25mg/L,在此继代培养基上,经过3～5次继代后可以得到4种不同类型的愈伤组织。

Taking Lonicera japonica Thunb. ＇Jinfeng No. 1＇ as material, the effect of different explants and different concentrations of plant growth regulators treatments on callus induction and subculture were studied. The results showed that the callus induction of the best explants was lower buds,and the best induction medium was MS＋NAA 2. 0 rag/L＋ 6-BA 0. 01 mg/L＋ 2,4-D 0. 5 rng/L, the inducing ratio could reach 100 % ; the callus grew faster, plenty, moistly, loose, colors brightly. The best subculture medium for callus was MS＋6-BA 2 mg/L＋KT 0. 75 mg/L＋NAA 0. 25 rag/L, 4 kinds of callus could be obtained after subcultured for 3-5 generations.