绿潮漂浮种的一种营养增殖方式的初步研究

Preliminary studies on certain autologous nutrition proliferation pattern of floating species in green tide

对所采集筛选的漂浮绿藻进行了定性和定量培养实验,并对筛选的绿潮漂浮种进行了ITS序列分析。根据实验结果,并未见有孢子或配子产生;对漂浮种的定期形态学显微观察也未见有成熟孢子囊或配子囊形成,但藻体的生物量一直处于增加状态。在实验条件下,生物量在30 d内增加约31倍。ITS序列系统进化树显示,QF1和QF2均被分在LPP复合体中,且亲缘关系较近,应属同一种类,但在外形上相差甚大。藻体的生长增殖一般情况下被并入自然生长,属于藻体自身长大。但实验藻体如其他绿潮优势种一样,均具有大量的分枝。分枝在从母体断裂或脱落后,成为新的母体进而进行增殖。这应当作为自体营养繁殖而成为绿潮漂浮种的另一种增殖方式,也是漂浮绿藻在适宜条件下生物量急剧增加的重要原因。

The acquisitions of green tide floating algae were screened and used into qualitative and quantitative culture experiment.Then ITS sequences analysis was carried out.According to the results of the experiments,neither spores nor gametes production was observed according to the regularly morphological or microscopic observation,as well as mature sporangium and gametangium.But on experimental conditions,algae biomass was increasing,and the biomass increased about 31 times in 30 days.The ITS sequence evolutionary tree shows that QF1 and QF2 were all included in LPP complex clade and had close genetic relationship.This showed they should belong to a same species.But the forms of the two differed from each other.The growth and proliferation of algae were usually incorporated into natural growth which was frond-self growing up.As other dominant floating species,the algae in our experiment had plenty of branches.When branches fell off or broke,a new alga formed and began multiplying.This should be considered as autologous nutrition proliferation pattern of green tide algae which belonged to another way of proliferation.This self-proliferation of green algae was another important reason of sharp biomass increasing of green tide on suitable conditions.