干露下三疣梭子蟹家系间血淋巴免疫因子活性差异分析

Analysis of the activity differences of immune factors among different parentages in Portunus trituberculatusat different desiccation temperatures

分别设置了10℃、20℃和30℃3个温度干露条件,比较了不同三疣梭子蟹家系血淋巴酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)、一氧化氮(NO)含量、过氧化物酶(POD)等5种非特异免疫因子活性随干露时间延长的变化情况。结果表明,酸性磷酸酶(ACP)指标在3个家系间没有差异,碱性磷酸酶(AKP)、过氧化氢酶(CAT)、一氧化氮(NO)含量和过氧化物酶(POD)在3个家系间存在着差异,其中一氧化氮含量(NO)可以作为指示家系是否具有耐干露特性的一个指标。在此基础上,进一步探讨了这些差异的原因及在培育三疣梭子蟹耐干露新品种上的意义。

The swimming crab Portunus trituberculatus（Crustacea： Decapoda： Brachyura）,is an important commercial crab,widely distributed in the coastal zones of Japan,Korea,China and Southeast Asia countries.In this paper,the activity difference of five immune factors among different parentages of P.trituberculatus following the time extension were compared at different desiccation temperature,10 ℃,20 ℃ and 30 ℃.The five immune factors were acid phosphatase（ACP）,alkaline phosphatase（AKP）,catalase（CAT）,nitrogen monoxide（NO）,peroxidase（POD）,respectively.The results showed that no significant difference was found among the parentages for the enzyme activity of ACP,but there existed some differences,more or less,for the other immune factors.The profile of the content of NO following the time extension has the same trend with the ability of the desiccation tolerance in three parentages,and this suggests that the content of NO can be used as a marker of desiccation tolerance for the inbreeding of P.trituberculatus.