茵陈蒿方传统与现代浸提方法对比

Comparison of Traditional and Modern Extraction Methods for Yinchenhao Prescription

目的:对比茵陈蒿方传统与现代浸提方法,确定最佳提取工艺。方法:以茵陈蒿方传统煎煮法为基础,采用紫外分光光度法,以总绿原酸、总环烯醚萜苷、总蒽醌及总多糖的综合评分为指标,通过单因素试验和正交试验优化茵陈蒿方的提取工艺。结果:最佳提取工艺为加12,8,6倍量水提取3次,提取时间分别为1,1,0.5 h;再加6倍量80%乙醇提取2次,每次0.5 h。结论:不同提取方法对茵陈蒿方有效成分含量有明显影响,两步提取法较其他方法更能充分提取其有效成分,且方法稳定可行,为该方合理应用和进一步研究提供实验依据。

Objective： To determine optimum extraction technology by comparing traditional and modern extraction methods of Yinchenhao prescription. Method： Based on traditional decoction of Yinchenhao prescription, the content of index components were determined by UV spectrophotometry, with composite score of total chlorogenic acid, total iridoid glycosides, total anthraquinones and total polysaccharides as index, extraction technology of Yinchenhao prescription was optimized by single factor test and orthogonal test. Result： Optimum extraction process was as following：extracted 3 times with 12, 8, 6 times the amount of water, extraction time of 1, 1, 0.5 h, respectively;Then extracted 2 times with 6 times the amount of 80% ethanol, 0.5 h per time. Conclusion： Different extraction methods had significant effects on the content of active ingredients in Yinchenhao prescription, two-step extraction method was better than other methods in extracting active ingredients from Yinchenhao prescription, and it was stable and feasible, it could provide experimental basis for reasonable application and further study of in Yinchenhao prescription.