复方制剂中洋金花药材的筛选和质量控制

Component Analysis and Content Comparison in Daturae Flos for the Control of Quality in Compound Preparation

目的:对不同产地洋金花中东莨菪碱及其他特征成分的含量进行比较分析,为药材选择和制剂质量控制提供参考。方法:采用高效液相色谱法,对色谱图进行分析,并以氢溴酸东莨菪碱为对照品,对各峰含量及百分率进行分析。结果:不同产地间洋金花中东莨菪碱的含量差异较大,海南的洋金花中东莨菪碱含量是广西的1.953倍;其他特征成分的含量也有较大差异,以5个特征成分的总和计算的百分比含量中,海南的洋金花是广西的1.687倍。结论:应用高效液相含量测定及指纹图谱的方法对有毒中药洋金花的质量进行控制是可行的。

Objective： To make a comparative study of daturae flos from different habitats. Method： HPLC was used to determine the content of scopolamine and other characteristic compositions. Result： The content of scopolamine and other composition in daturae flos from different habitats were more different, the content of scopolamine in daturae flos from Hainan province was 1.953 times that from Guangxi province.There is also a big difference in other compositions, the total quantity of five characteristic compositions of Hainan province was 1.687 times that from Guangxi province. Conclusion： The method can be used for the quality control of daturae flos, and the analysis of fingerprint is workable for quality evaluation.