金莲花不同花部对总黄酮的贡献度研究

Investigation on the Contribution of Floral Parts to Total Flavonoids of Trollius chinensis

目的:测定金莲花不同花部总黄酮的含量及质量分数,确定各花部对金莲花总黄酮的贡献度。方法:比色法测定金莲花不同花部中总黄酮的含量;质量分析法测定不同花部的质量分数;采用数学方法计算各花部对金莲花总黄酮的贡献度。结果:金莲花各花部的总黄酮含量为:花萼(6.18%)、花柄(2.84%)、花冠(2.22%)、子房(1.52%)、花蕊群(1.07%)。金莲花各花部的质量分数为:花萼(41.90%)、花蕊群(22.40%)、子房(15.29%)、花冠(10.24%)、花柄(10.17%)。结论:金莲花5个花部对总黄酮的贡献度依次为:花萼(72.00%)、花柄(8.19%)、花蕊群(6.85%)、子房(6.54%)、花冠(6.42%),花萼对总黄酮的贡献度最大。

Objective： To ascertain the contribution of different floral parts to the total flavonoids of Trollius chinensis and the mainly enriched part of total flavonoids. Method： Colorimetric method was employed to assay the content of total flavonoids of different floral parts and gravimetric analysis method was used to determine the mass fraction of floral parts.The contribution degree was calculated with special algorithm. Result： The content of total flavonoids in each floral part is as follows： calyx（6.18%）, stalk（2.84%）, corolla（2.22%）, ovary（1.52%）, and stamen and pistil（1.07%）.The mass fraction of each floral part is as follows： calyx（41.90%）, stamen and pistil（22.40%）, ovary（15.29%）, corolla（10.24%）, and stalk（10.17%）. Conclusion： The contribution of five floral parts to total flavonoids is as follows： calyx（72.00%）, stalk（8.19%）, stamen and pistil（6.85%）, ovary（6.54%）, and corolla（6.42%）.Calyx is the most enriched part of total flavonoids.