HPLC同时测定蒙药胃舒安胶囊中大黄素、大黄酸、大黄酚的含量

Determination of Rhien,Emodin and Chrysophanol in Weishuanjiaonang by HPLC

目的:建立蒙药胃舒安胶囊中3种蒽醌成分大黄素、大黄酸、大黄酚含量的方法。方法:采用HPLC法,使用色谱柱VP-ODS(4.6 mm×150 mm,5μm),以甲醇-0.1%磷酸水溶液(85∶15)为流动相,检测波长254 nm,流速0.6 mL.min-1。结果:此方法能使样品中的3种蒽醌类成分得到良好分离且线性关系良好,平均回收率为大黄酸98.76%,RSD 0.56%;大黄素97.22%,RSD 0.33%;大黄酚97.53%,RSD 0.76%。结论:方法简单、快速、结果满意,可用于作为胃舒安胶囊质量控制方法之一。

Objective： To establish a method for the determination of rhien, emodin and chrysophanol in Weishuanjiaonang by HPLC. Method： Rhien, emodin and chrysophanol in Weishuanjiaonang was determined by HPLC on VP-ODS （4.6 mm×150 mm, 5 μm） column with methyl petchloric acid （0.1%）（85： 15） as a mobile phase and the detective wavelength was 254 nm, the flow rate is 0.6 mL·min-1. Result： The good linear relationship of rhien, emodin and chrysophanol in Weishuanjiaonang.The average recovery of rhien was 98.76%, RSD 0.56%;The average recovery of emodin is 97.22%, RSD 0.33%.The average recovery of chrysophanol was 97.53%, RSD 0.76%. Conclusion： The method is simple, rapid and gives satisfying results.It can be suitable for quality control of Weishuanjiaonang.