高效液相色谱法测定枳实-白芍配伍的化学成分变化

Changes of Chemical Components in Compatibility of Citrus Aurantium with Paeoniae Radix Alba

目的:研究枳实和白芍配伍后化学成分在质和量上的变化。方法:采用HPLC比较枳实-白芍单煎液与合煎液中各成分的变化,色谱柱为Syncronis-C18(4.6 mm×250 mm,5μm),柱温25℃,流动相乙腈-0.3%磷酸水溶液,检测波长230nm。结果:方法学考察表明,所用色谱条件符合定性和定量研究要求,确定了19个共有峰,并初步对各共有峰进行归属判断,其中8个成分含量增加,而9个成分含量降低。枳实-白芍配伍后化学成分没有发生质的改变,但含量上发生了显著变化。结论:中药复方的配伍并不是单味中药的简单相加,而是有一定科学依据的。

Objective： To study the changes of quality and quantity of chemical components in compatibility of Zhishi Shaoyao Powder. Method： HPLC was used to determine and analyze the peaks change of chemical components of single and co-decoction.The determination of Fructus Aurantii Immaturus and Radix Paeoniae Alba was performed on Syncronis-C18（4.6 mm×250 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.3% phosphoric-water at detection wavelength of 230 nm. Result： Nineteen peaks were selected as the fingerprint peaks.It was found that eight ingredients were increased and nine were decreased.This indicated that some changed more in all chemical components of co-decoction than single decoction. Conclusion： The compatibility of traditional Chinese medicine is not the simple adding of single herbs, but it includes some scientific base.