益气解毒方对大鼠局灶性脑缺血氧化应激的影响

Effect of Yiqi Jiedu Formula on Oxidative Stress in the Focal Cerebral Ischemia Rat

目的:研究中药益气解毒方对大鼠局灶性脑缺血后氧化应激损伤的影响。方法:取健康雄性SD大鼠60只,随机分为6组:假手术组、模型组、阳性药银杏叶提取物组(4 mg.kg-1)、益气解毒高、中、低剂量组(25,5,1 mg.kg-1)。给药组于手术麻醉前10 min灌胃给药,用线栓法制作大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,12 h后取血清和缺血脑组织制备组织匀浆液,用酶标仪定量检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、单胺氧化酶(MAO)、髓过氧化物酶(MPO)。结果:与假手术组比较,模型组脑组织SOD和GSH-Px活性明显降低(P<0.01),MDA含量和MAO,MPO活性明显升高(P<0.05)。与模型组比较,益气解毒方高、中、低剂量组、阳性药组SOD和GSH-Px显著性升高(P<0.01),MAO,MDA和MPO显著性降低(P<0.05,P<0.01)。结论:益气解毒方对大鼠局灶性脑缺血有保护作用,其作用机制可能与抗氧化应激作用有关。

Objective： To study effect of Yiqi Jiedu formula on oxidative stress in rat focal cerebral ischemia injury model. Method： SD rats were randomly divided into Yiqi Jiedu groups（25, 5, 1 mg·kg-1）, the model group, sham operation group, and positive control group（4 mg·kg-1）.Rsts were given drugs before anesthesia and establish middle cerebral artery occlusion （MCAO） model.The microplate reader was used to detect superoxide dismutase（SOD）, malondialdehyde（MDA）, glutathione peroxidase（GSH-Px）, monoamine oxidase（MAO） and myeloperoxidase（MPO） in the serum and cerebral homogenate. Result： Compared with the sham group, in model group SOD and GSH-Px decreased（P〈0.01）, the content of MDA and the activity of MAO and MPO increased（P〈0.05）.Compared with the model group, SOD and GSH-Px of Yiqi Jiedu groups and positive group were significantly higher（P〈0.01）, however MAO, MDA and MPO were decreased （P〈0.05, P〈0.01）. Conclusion： Yiqi Jiedu formula can exert protection antion in focal cerebral ischemia rats, the mechanism may be related to raising efficiency and reducing toxicity.