益心脑滴丸对心肌缺血再灌注损伤的保护作用

Protective Effect of Yixinnao Dripping Pills on Myocardial Ischemia Reperfusion Injury in Rats

目的:观察益心脑滴丸对Wistar大鼠心肌缺血再灌注损伤的保护作用。方法:将健康成年大鼠随机分为6组:假手术(sham)组、缺血再灌注损伤(I/R)组、地奥心血康(DAXXK,0.07 g.kg-1)组、益心脑滴丸高(YXNH,3 g.kg-1)、中(YXNM,1.5 g.kg-1)、低剂量(YXNL,0.75 g.kg-1)组,连续预防口服给药7 d。采用结扎左冠状动脉前降支30 min、再灌注60 min的方法建立大鼠心肌缺血再灌注损伤模型。动态观察血流动力学各检测指标,心电图(ECG)变化,同时监测室性心律失常的变化。再灌注结束后,测定血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,测量心肌梗死面积。结果:与sham组比较,I/R组心率(HR)、左室收缩压(LVSP)、左室压最大上升和下降速率(±dp/dtmax)明显降低,左室舒张末期压(LVEDP)显著升高(P<0.01);SOD活性明显降低,MDA含量明显升高(P<0.01),且梗死区/缺血危险区(An/AAR)显著增大(P<0.05)。与I/R组相比,YXNH能明显升高HR,LVSP,±dp/dtmax,降低LVEDP,(P<0.01或P<0.05),降低室性心律失常的持续时间(P<0.01),增高SOD活性,降低MDA含量(P<0.01),明显减小An/AAR(P<0.01),其作用强度与DAXXK相似;YXNM和YXNL对I/R有保护作用,但没有DAXXK明显。结论:益心脑滴丸对I/R具有保护作用,其机制可能与改善心肌功能和抗氧自由基损伤有关。

Objective： To observe the protective effect of Yixinnao dripping pills （YXN） on myocardial ischemia reperfusion injury （I/R） in Wistar rats. Method： The rats were randomly divided into six groups： sham-operated （sham） group, I/R group （I/R）, Diao Xinxuekang（DAXXK, 0.07 g·kg-1） treated group, high, medium and low dose groups pretreated by YXN （3, 1.5, 0.75 g·kg-1）, each group orally administered 7 days.Then, the left anterior descending coronary artery was ligated for 30 min, followed by 60 min of reperfusion to establish the rat myocardial ischemia-reperfusion injury model.Hemodynamic and electrocardiogram（ECG） were observed continuously, and ventricular arrhythmias were measured.At the end of reperfusion, the serum malondialdehyde （MDA） concentration and superoxide dismutase（SOD）activity were measured.The myocardial infarct size was measured after reperfusion. Result： Heart rate（HR）, left ventricular systolic pressure（LVSP）, the maximum rise rate of left ventricular pressure （＋dp/dtmax） and the maximum descending of left ventricular pressure （-dp/dtmax） and activity of SOD decreased obviously in I/R group compared with those in sham group; MDA content increased obviously （P〈0.01） and the area of necrosis（An）/area at risk（AAR） was significantly elevated （P〈0.05） in the I/R group. Compared with I/R, YXNH could significantly improve the HR, LVSP, ±dp/dtmax and reduce the LVEDP（P〈0.01 or P〈0.05）, decrease the lasting time of ventricular arrhythmias（P〈0.01）; the activity of SOD was obviously increased, and MDA content was decreased （P〈0.01）; the An/AAR was smalle r（P〈0.01）.The effect was similar to that of DAXXK.YXNM and TXNL also had protective effect on I/R in rats, but their effect was not as good as DAXXK. Conclusion： YXN has protective action on I/R in rats, which may be related to promoting the functions of myocardium and enhancing the antioxidant ability.