加味苓桂术甘汤对代谢综合征大鼠血清抵抗素、脂联素、胰岛素、胰岛素抵抗的影响

Effects of Reformatted Linggui Zhugan Decoction on the Level of Serum Resistin,Adiponectin,Insulin and Insulin Resistance in Metabolic Syndrome Model Rats

目的:探讨加味苓桂术甘汤对代谢综合征模型大鼠抵抗素、脂联素、胰岛素和胰岛素抵抗指数的影响。方法:取SPF级雄性大鼠,随机分为正常对照组,高脂高盐(模型)组,二甲双胍(降糖)组,阿托伐他汀(降脂)组,加味苓桂术甘汤(试药)高(18 g.kg-1)、中(12 g.kg-1)、低(6 g.kg-1)剂量组,每组动物10只。除正常对照组外,各组大鼠均以高脂高盐饲料喂养。并于模型制作当日分别ig给予药物治疗,每日1次,连续8周。正常对照组、模型组给予同样量的生理盐水。采用酶联免疫吸附分析方法测定血清抵抗素、脂联素、胰岛素水平,计算胰岛素抵抗指数。结果:模型组抵抗素、脂联素、胰岛素水平及胰岛素抵抗指数与正常组比较均有显著性差异(P<0.05)。加味苓桂术甘汤高、中、低剂量组均能显著降低代谢综合征模型大鼠血清抵抗素、升高脂联素含量(P<0.05或P<0.01),均能显著降低代谢综合征模型大鼠胰岛素水平及降低胰岛素抵抗指数(P<0.05或P<0.01)。结论:加味苓桂术甘汤对代谢综合征模型大鼠抵抗素、脂联素、胰岛素水平及胰岛素抵抗均有调节作用。提示加味苓桂术甘汤有调节脂肪和糖代谢的作用。

Objective： To explore the effects of reformatted Linggui Zhugan decoction on metabolic syndrome. Method： Seventy male specified-pathogens free （SPF） Wistar rats, aged 3 weeks, were divided into seven groups in randomized manner： control group treated with distilled water diet; high fat （49% lard） and high salt （4% NaCl, model group）; high fat and salt group treated with atovastatin （lipid-lowering group）; high fat and salt group treated with metformin （hypoglycemic group）; high fat and salt group treated with reformatted Linggui Zhugan decoction large dose, middle dose and small dose groups.The rats of these groups were taken in high fat and high salt except control group.And rats of each group were administrated by gavge at the same time once every day for 8 weeks.Among them, lipid-lowering group rats were given 8 mg·kg-1 atovastatin; hypoglycemic group rats 670 mg·kg metformin; reformatted Linggui Zhugan decoction large dose group, middle dose group and small dose group rats 18, 12, 6 g·kg-1 reformatted Linggui Zhugan decoction concentrated solution differently.Control group and model group rats were given normal salt administration by gavage equal content.The level of serum resistin, adiponectin and insulin were detected by enzyme-linked immunosorbent assay （ELISA）.And insulin resistance （HOMA-IR） index was counted. Result： The level of resistin, adiponectin, insulin was of a very significant difference after reformatted Linggui Zhugan decoction were given for 8 weeks, compared with control group and model group （all P〈0.01）.The level of insulin and HOMA-IR index was not significantly difference compared with the control group, lipid-lowering group and hypoglycemic group, but there was a significant difference compared with the model group （all P〈0.01）. Conclusion： The reformatted Linggui Zhugan decoction can regulate metabolic syndrome, adiponectin, insulin and HOMA-IR index.It is suggested that reformatted Linggui Zhugan decoction have effect on regulation fat and saccharide metabolism.