转基因猪外源hDAF基因的定位

The Localization of Exogene hDAF on Transgenic Pigs

应用非同位素原位杂交技术研究转基因猪外源ｈＤＡＦ基因在受体基因组染色体上的整合及其定位．结果表明，外源基因在转基因猪中呈随机多位点整合，转基因分别定位在２Ｐ、６Ｐ１４、７Ｐ１１－Ｐ１３、７ｑ２６、１１ｑ１２、１３ｑ４４－ｑ４８、１４ｑ２６．

In order to determine the integration and localization of hDAF transgene on transgenic pig chromosomes, nonisotope in situ hybridization technique was applied. The results indicate that exogene hDAF was limitedly distributed on chromosomes of transgenic pigs and localized on 2P, 6P14, 7P11--p13, 7q26, 11q12, 13q44--q48, 14q26, respectively.