期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

胎盘11β-HSD2表达与皮质醇代谢水平的关系及其对子痫前期的相关性研究 被引量：3

Correlation study on the expression of 11β-HSD2 in placenta and cortisol level of maternal blood in preeclampsia
原文传递
导出
摘要 目的:探讨胎盘11β-HSD2和母体皮质醇水平在不同的子痫前期病理状态下的表达及其可能机制。方法:收集15例早发型重度子痫前期患者(EOSPE组)、13例晚发型重度子痫前期患者(LOSPE组)和20例正常妊娠孕妇(NP组)的血清及胎盘组织,用酶化学发光分析仪检测血清中游离皮质醇的浓度,采用免疫组化超敏即用型二步法观察胎盘11β-HSD2在合体滋养层细胞的表达,采用实时荧光定量PCR法检测胎盘组织11β-HSD2的mRNA表达。结果:正常妊娠、早发型、晚发型重度子痫前期组的血清游离皮质醇水平分别为(728.05±155.91)nmol/L、(619.80±180.57)nmol/L、(750.53±153.80)nmol/L,组间比较差异无统计学意义(P>0.05)。免疫组化胎盘11β-HSD2测量平均光密度值正常妊娠、早发型、晚发型重度子痫前期组分别为0.12±0.06、0.07±0.02、0.08±0.04,组间比较差异有统计学意义(P<0.05)。实时荧光定量PCR测量胎盘11β-HSD2的mRNA表达水平在早发型、晚发型重度子痫前期组和正常妊娠组分别为:3.16±1.21、4.86±1.43、5.23±2.20,组间比较差异有统计学意义(P<0.05)。结论:胎盘11β-HSD2可能灭活皮质醇从而保护胎儿,11β-HSD2表达减少与SPE发病有关。 Objective： To explore the expression of 11β - Hydroxysteroid dehydrogenase 2 （11β - HSD2） and cortisol level in pregnant women with different types of preeclampsia. Methods： The samples of serum and placenta were obtained from normal pregnanl women （NP, n = 20）, early - onset severe preeclampsic women （EOSPE, n = 15） and late - onset severe preeclampsic women （ LOSPE. n = 13） . The concentration of free cortisol in serum was detected by the method of chemiluminescence. Immunohistochemistry was used to detect the expression of 11β - HSD2 on placenta; the mRNA expression of 11β - HSD2 in placenta were evaluated by real time fluorescenco quantitative PCR. Results： Serum levels of cortisol in NP group, EOSPE group and LOSPE group were （728.05 ± 155.91 ） nmol/1,, （619. 80 ±180. 57） nmol/L, （750. 53 ± 153.80） nmol/L, shown no statistical differences （P 〉0. 05） . The mean optical density value in NP group （0. 12 ±0. 06） , EOSPE group （0. 07±0. 02） , LOSPE group （0. 08 ±0. 04）, shown statistical differences （P 〈0. 05） . The mRNA expression level of 11β -HSD2, which was 3.16± 1.21 in EOSPE group and 4. 86 ± 1.43 in LOSPE group respectively, was significantly lower than 5.23 ± 2.20 in NP group （ P 〈 0. 05 ） . Conclusion ： The lower expression level of 11β- HSD2 in placenta may contribute to the higher cortisol level in fetal of preeclampsic women and play an important role in the pathogenesis of the disease.
作者 姚锋祥 汤彪 黄引平
机构地区 温州医学院附属第一医院产科
出处 《中国妇幼保健》 CAS 北大核心 2013年第8期1336-1339,共4页 Maternal and Child Health Care of China
基金 国家自然科学基金项目〔81070510〕
关键词 胎盘11β-羟基类固醇脱氢酶2 胎盘 子痫前期 皮质醇 11β- Hydroxysteroid Dehydrogenase2 Placenta Preeclampsia Cortisol
分类号 R714.245 [医药卫生—妇产科学]
  • 相关文献

参考文献10

  • 1Quinkler M, Oelkers W, Diederich S. Clinical implications of glucocorticoid metabolism by 11 [3 -hydroxysteroid dehydrogen- ases in target tissues [ J] . European Journal of Endocrinolo- gy, 2001, 144:87.
  • 2Atanas G, Irena D, Lyubomir Get al. Impaired protein stabil- ity of 11 - hydroxysteroid dehydrogenase type 2 : a novel mechanism of apparent mineral corticoid excess[J] . J Am SocNephrol, 2007, 18 (4): 1262.
  • 3Challis JRG, Kristin Connor. Glucocorticoids, 1113 - Hydrox- ysteroid Dehydrogenase: mother, fetus, or both? [J] . En- docrinology, 2009, 150 (3): 1073.
  • 4Dy J, Guan H, Sampath - Kumar R et al. Placental 11 beta - hydroxysteroid dehydrogenase type 2 is reduced in pregnancies complicated with idiopathic intrauterine growth restriction: evi- dence that this is associated with an attenuated ratio of corti- sone to cortisol in the umbilical artery [ J ]. Placenta, 2008, 29 (2): 193.
  • 5French NP, Hagan R, Evans SF et al. Repeated antenatal cor- ticosteroids: size at birth and subsequent development [J] .Am J Obstet Gynecol, 1999, 180:114.
  • 6School E, Girstl M, Frobenius Wet al. Course of placental 11 - hydroxysteroid dehydrogenase type 2 and 15 - hydroxypros- taglandin dehydrogenase mRNA expression during human gesta- tion [J] . Eur J Endocrinol, 2001, 145:187.
  • 7Hardy DB, Yang K. The expression of 11 - hydroxysteroid de- hydrogenase type 2 is induced during trophoblast differentia- tion: effects of hypoxia [J]. J Clin Endoerinol Metab, 2002, 87 : 3696.
  • 8Myriam Aufdenblatten, Marc Baummm, Luigi Raio et al. Prema- turity is related to high placental cortisol in preeclampsia [J].Pediatr Res, 2009, 65 (2): 198.
  • 9Mericq V, Medina P, Kakarieka E et al. Differences in ex- pression and activity of 11 β - hydroxysteroid dehydrogenase (11 β- HSD) type 1 and 2 in human placentas of term preg- nancies according to birth weight and gender [ J] . European Society of Endocrinology, 2009, 9:101.
  • 10Bettina Lanz, Bert Kadereit, Samuel Ernst et al. Angiotensin Ⅱ regulates 11 β - hydroxysteroid dehydrogenase type 2 via AT2 receptors [J] . Kidney International, 2003, 64, 970.

同被引文献48

  • 1李晖,李清红,朱忠良,陈蕊,成大欣,蔡青,贾宁,宋亮.产前束缚应激子代大鼠海马神经颗粒素表达降低[J].生理学报,2007,59(3):299-304. 被引量：5
  • 2Xu XF, Gu WZ, Wu XL, et al.Fetal pulmonary vascular remodeling in a rat model induced by hypoxia and indomethacin[J].J Matern Fetal Neonatal Med, 2011, 24(1):172-182.
  • 3Das R, Balonan L, Ballard HJ, et al.Chronic hypoxia inhibits the antihypertensive effect of melatonin on pulmonary artery[J].Int J Cardiol, 2008, 126(3): 340-345.
  • 4Dy J, Guan H, Sampath-Kumar R, et al.Placental 11beta-hydroxysteroid dehydrogenase type 2 is reduced in pregnancies complicated with idiopathic intrauterine growth restriction:evidence that this is associated with an attenuated ratio of cortisone to cortisol in the umbilical artery[J].Placenta, 2008,29(2): 193-200.
  • 5Ostreicher I, Almeida JR, Campean V, et al.Changes in 11beta-hydroxysteroid dehydrogenase type 2 expression in a low-protein rat model of intrauterine growth restriction[J].Nephrol Dial Transplant, 2010, 25(10): 3195-3203.
  • 6Garbrecht MR, Schmidt TJ, Krozowski ZS, et al.11Beta-hydroxysteroid dehydrogenase type 2 and the regulation of surfactant protein A by dexamethasone metabolites[J].Am J Physiol Endocrinol Metab, 2006, 290(4): E653-E660.
  • 7Campino C, Carvajal CA, Cornejo J, et al.11β-hydroxysteroid dehydrogenase type-2 and type-1(11β-HSD2 and 11β-HSD1) and 5β-reductase activities in the pathogenia of essential hypertension[J].Endocrine, 2010, 37(1): 106-114.
  • 8Ong SLH, Whitworth JA.How do glucocorticoids cause hypertension: role of nitric oxide deficiency, oxidative stress,and eicosanoids[J].Endocrinol Metab Clin North Am, 2011,40(2): 393-407.
  • 9Dodt C, Wellhoner JP, Schutt M, et al.Glucocorticoids and hypertension[J].Internist(Berl), 2009, 50(1): 36-41.
  • 10Hadoke PWF, Christy C, Kotelevtsev YV, et al.Endothelial cell dysfunction in mice after transgenic knockout of type 2, but not type 1, 11β-hydroxysteroid dehydrogenase[J].Circulation, 2001,104(23): 2832-2837.

引证文献3

二级引证文献8

中国妇幼保健
2013年 第8期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部