响应面优化离子液体体系酶催化合成阿魏酰基甘油酯

Optimization of lipase-catalyzed synthesis of feruloyl glycerols in ionic liquid using response surface methodology

以离子液体EMIMTF2N为溶剂体系,采用Box-Behnken响应面法对Novozym435催化阿魏酸乙酯和甘油酯化合成阿魏酸甘油进行了研究。结果表明:反应温度、体系酶浓度、反应时间及它们之间的交互作用对阿魏酸乙酯转化率有着极显著的影响(P<0.001);底物摩尔比对阿魏酸乙酯转化率不具显著影响,但是与其他因素的交互作用却有着高度显著的影响(P<0.01)。经优化得到最佳工艺条件为:反应温度80℃、体系酶浓度60mg/mL、反应时间5.0h、底物比1.4:1(mol/mol),EF转化率为99.47%。

Using ionic liquid EMIMTF2N as reaction media, feruloyl glycerols was synthesized from glycerol and ethyl ferulate （EF） catalyzed by Novozym 435. Reaction conditions were optimized by Box-Behnken design and response surface methodology （RSM）. The results showed that the reaction temperature, reaction time, enzyme load and their interactivity strongly affected EF conversion （P〈0.001）. Substrate molar ratio did not have a significant （P〉0.05） effect on conversion, however, its interactivity with other parameters （P〈0.01） were opposite. The optimum conditions were as follows： reaction temperature 80 ℃, enzyme load 60 mg/mL, reaction time 5.0 h, substrate ratio 1.4：1 （mol/mol）. EF conversion was 99.47% under these conditions.