西兰花籽中萝卜硫苷的提取、分离及结构鉴定

Extraction,Purification and Identification of Gulcoraphanin from Broccoli Seeds

研究一种从西兰花籽中提取分离萝卜硫苷(4-甲基亚磺酰丁基硫代葡萄糖苷)的方法。用甲醇溶液从西兰花籽中提取硫苷,经弱阴离子交换树脂纯化得到萝卜硫苷粗品,通过半液相制备色谱进一步分离纯化,制得萝卜硫苷纯品。利用液-质联用、核磁共振、红外光谱检测、紫外光谱检测等方法对其进行结构确定,结果符合萝卜硫苷的分子结构。此方法适用于萝卜硫苷的简单有效提取。

A method to isolate and purify gulcoraphanin（4-methylsufinylbutyl glucosionlate） from broccoli seeds was reported.Gulcoraphanin was extracted from broccoli seeds with a mixture of methanol and water,and purified by DEAE-Sephadex A25 anion exchange column chromatography.Pure glucoraphanin was obtained by preparative HPLC,and identified by HPLC-MS,NMR,IR and UV spectroscopy.The results were consistent with the molecular structure of glucoraphanin.The described method is rapid,robust and suitable for preparing pure glucoraphanin.