β-胡萝卜素降解葡萄球菌化学合成培养基营养素的研究

Screening of Nutrients in Chemically Defined Medium for β-Carotene-Degrading Staphylococcus sp.

目的:探索适于β-胡萝卜素降解葡萄球菌生长的最佳化学合成培养基配方。方法:先确定培养基成分,再利用单一成分缺失方法确定β-胡萝卜素降解葡萄球菌的必需营养素。结果:K2HPO4、NaNO3、Pro、Arg、Val、Gly、Try等物质是此菌体生长必需的营养素。结论:经筛选合成的完全化学合成培养基可以用于此β-胡萝卜素降解葡萄球菌的培养,培养物具有降解类胡萝卜素能力。

Objective：To explore the optimum synthetic medium suitable for β-carotene-degrading Staphylococcus sp..Methods：The medium composition was determined and essential nutrients for β-carotene-degrading Staphylococcus sp.were chosen by single component deficiency method.Results：K2HPO4,NaNO3,proline,arginine,valine,glycine and tryptophan were essential for the growth of β-carotene-degrading Staphylococcus sp..Conclusion：The optimized synthetic medium is suitable for the cultivation of β-carotene-degrading Staphylococcus sp.to produce β-carotene-degrading materials.